Testing Antimicrobials Using MBEC

Minimum Biofilm Eradication Concentration (MBEC) assay is used to test compounds for their ability to penetrate bacterial biofilm. Biofilms are formed by many species of bacteria and some fungi for cell adherence and protection. By creating biofilms, bacteria can form colonies almost anywhere, including the human body and medical equipment. Biofilms produced by bacteria render most of currently available antibiotics ineffective by creating a “shield” through which antibiotics cannot penetrate through.

At EP, we have the resources to determine the efficacy of an antimicrobial test agent against our large, ready-to-go inventory of bacterial clinical isolates and fungal sample biofilm using the efficient and accurate MBEC (minimum biofilm eradication concentration) Assay.

For this assay, the test organisms are first grown in a 96-well plate with pegs on the lid filled with growth medium to inoculate the pegs. The lid is then transferred to another 96-well plate filled with the antimicrobial test agents and incubated. After this, from the inspection of the plate for growth, the MBEC value, or minimum concentration which destroys biofilm, can be determined.

Below is a representation of a MBEC assay that uses a Calagary Biofilm Device.

Biofilm MBEC Illustration