Minimum Biofilm Eradication Concentration (MBEC) assay is used to test compounds for their ability to penetrate microbial biofilms. Biofilms are formed by many species of bacteria and some fungi for cell adherence and protection. By creating biofilms, bacteria can form colonies almost anywhere, including in the human body and on medical equipment. Biofilms produced by bacteria render most currently available antibiotics ineffective by creating a barrier through which these antibiotics cannot penetrate through and therefore attack the bacteria.
In this assay, the biofilm-forming test organisms are first grown in a 96-well plate, where pegs on the lid are dipped in growth medium to inoculate the pegs. The lid is then transferred to another 96-well plate filled with the antimicrobial test agents and incubated. After this, from the inspection of the plate for growth, the MBEC value, or minimum concentration which destroys biofilm, can be determined.
Below is a representation of a MBEC assay that uses a Calagary Biofilm Device.