Biofilms are formed when free-swimming, planktonic microbes adhere to one another and to a surface, and are often resistant to antibiotics. To test this antibiotic resistance, Emery Pharma offers a microplate peg lid-based Minimum Biofilm Eradication Concentration (MBEC) assay and crystal violet biomass staining assay in 96-well microplate format.
MBEC assays can be used to determine the minimum concentration of a test compound that will penetrate and eradicate microbial biofilms. This assay also allows for consistent biofilm generation and evaluation of multiple test compounds over a range of concentrations. Both antiseptic (as described in ASTM E2799-17) and antibiotic compounds can be tested.
Crystal violet is a dye that stains both microbial cells as well as extracellular matrix, and is commonly used as a readout for biomass. At Emery Pharma, crystal violet staining can be performed in conjunction with the MBEC assay or as an independent assay to evaluate biofilm biomass.