What is Proteomics?
Proteomics is the study of the proteome, the entire set of proteins produced by a living system. It involves identification of proteins, investigation of protein abundances, the variations, and modifications therein, as well as insight into interacting partners and networks.
Practical applications of proteomics include
- New drug discovery
- Mechanism of action
- Protein identification
- Protein quantitation
- Interaction Proteomics and Protein Networks
- Expression Proteomics
At Emery Pharma, we have a comprehensive platform with advanced instruments including UPLC-HR MS, GC-MS, UPLC-QqQ-MS/MS, and NMR that can be applied to the analysis of a single protein or used for the large-scale analysis of a proteome including protein abundances, modifications, protein-protein interactions, and protein-compound interactions.
Our proteomics services support cancer research, diagnostic biomarker discovery, microbiology, antibody profiling, and drug discovery and development. We are happy to support our clients with our deep expertise across the proteomics pipeline.
Bottom-up and Top-down Proteomics
Proteomics is usually performed using two different strategies: bottom-up and top-down approaches.
Bottom-Up Approach to Proteomics
In the bottom-up approach, a crude protein mixture is subjected to proteolytic cleavage, and the peptide products are analyzed by mass spectrometry.
This approach routinely allows greater sequence coverage, resolution of sequence ambiguities and preservation of post-translational modifications. Peptides are more easily separated by LC as compared to intact proteins, and ionize and fragment more efficiently in the MS, making it a robust methodology for high-throughput analysis for identification and quantification of complex proteins mixtures.
Top-Down Approach to Proteomics
In the top-down approach, intact proteins are characterized by mass spectrometry without prior proteolysis.
This approach offers the ability to study protein isoforms, sequence variants, as well as significantly simplifies the sample preparation, data analysis, and quantitation process. This method is superior for especially low mass protein detection since the inability to generate sufficient tryptic peptides can be challenging for MS analysis.
Emery Pharma offers extensive experience in both bottom-up and top-down proteomics. Please inquire to discuss your proteomics projects, matrix compatibility, and choosing the right workflow.