The applications of HPLC encompass a broad range of fields and include testing the quality of products you use every day. At Emery Pharma we employ many different types of chromatographic techniques that go above and beyond HPLC, including ion-exchange (IEC), size exclusion (SEC), preparative (Prep-LC), and gas (GC).
Typical Examples of HPLC analyses include:
- Impurity analysis
- Degradation analysis
- Target characterization and identification
- Food/beverage ingredient characterization
- Peptide mapping and sequencing
- Sugar analysis
- Carbohydrate and fatty acid analysis
- Metabolomics
- Antibody analysis
- Purification
- Toxicology screening
- All non-volatile and many semi-volatile analytes including antibiotics, excipients, ions, lipids, biofuels, sugars, and surfactants.
Instrumentation:
Agilent 1200 Series High Performance LC
Agilent 1290 Infinity Series High Performance LC
Agilent 1200 Series Diode Array and Multiple Wavelength Detector SL, G1315C/D and G1365 C/D
Agilent 6400 Series Triple Quadrupole LC/MS System
Thermo Fisher Scientific Vanquish UHPLC
Thermo Fisher Scientific Vanquish Diode Array/Multiple Wavelength Detectors (VC-D11, VC-D12, VF-D11)
Thermo Fisher Scientific Vanquish Charged Aerosol Detector (VH-D20, VF-D20)
Thermo Fisher Scientific RefractoMax 520 Refractive Index Detector
Thermo Fisher Scientific, ISQ 7000 MS
Thermo Fisher Scientific, Trace 1300 Gas Chromatography/FID
Multiple mass libraries, including the one from NIST, from which we can search and provide quick identification for the components in these and other samples.
Refractive Index Detector (RI Detector) -It is used to measure the refractive index of an analyte relative to the solvent. It is often used as detector for high-performance liquid chromatography (HPLC) and size exclusion chromatography (SEC). It is mainly used when detecting analytes with limited or no UV absorption including chemical components such as alcohols, sugars, fatty acids, polymers, and carbohydrates.
Charged Aerosol Detector (CAD)- It is used to measure the amount of analyte in a sample by creating charged aerosol particles, which are detected using an electrometer. It is used as detector for HPLC and ultra-high-performance liquid chromatography (UHPLC). It is mainly used for analytes that cannot be detected using UV/Vis approaches.
UV-Vis Detector- It is used to measure the amount of ultraviolet or visible light that is absorbed by an analyte being eluted off the chromatography column. It is used as detector for HPLC, UHPLC and preparative HPLC. It is mainly used for development of active pharmaceutical ingredients, quantification of impurities, dissolution testing, and quantification of nucleic acids and proteins in pharmaceutical development.
Mass spectrometer (MS)- It is used to identify unknown compounds via molecular weight determination, quantify known compounds, and determine structure and chemical properties of molecules. The results are presented as a mass spectrum, a plot of intensity as a function of the mass-to-charge ratio. It is used as detector for HPLC and UHPLC. They are widely used for small molecules, oligonucleotides, lipids, glycans, proteins, peptides and determining the location of post translational modifications.
