“ Microbiology and cell biology laboratory expertise, from routine antimicrobial screening, to method development, to advanced Research & Development projects”
Microbiology and Cell Biology Laboratory Services
Emery Pharma offers a wide range of microbiology and cell biology services in our state-of-the-art laboratory with hundreds of bacterial strains including multidrug-resistant clinical isolates, ESKAPE pathogens, and multiple fungal strains available in our inventory for immediate testing. Our laboratory at Emery Pharma routinely performs microbiological testing and our Ph.D. scientists provide both recommendations for methods set up, such as suggesting microorganisms to test, and insightful and timely analysis of results.
Emery Pharma offers the following microbiological services:
Minimum Inhibitory Concentration (MIC) is a type of antimicrobial susceptibility assay. It is used to determine the lowest concentration of antimicrobial agent needed to inhibit visible growth of microorganisms in broth or agar. Emery Pharma offers a wide range of MIC assays, including broth macro- and microdilution, disk diffusion, and agar dilution.
The MBC and MFC Assay is used to determine the lowest concentration of antimicrobial agents that results in microbial death. The MBC is complementary to the MIC, and the MBC assay is normally performed after a macro- or microdilution MIC assay to determine if the antimicrobial agents are bactericidal or bacteriostatic.
The Time-Kill Kinetics Assay is used to assess the rate of killing of microorganism by an antimicrobial agent, which is done by measuring the number of viable microorganisms left at various time points after exposure to an antimicrobial agent. Emery Pharma offers time-kill assays for antiseptics (i.e. short intervals between time points on the order of minutes) as well as antibiotic test compounds (i.e. longer intervals between time points on the order of hours).
Biofilms are formed by microorganisms, including bacteria and fungi, for cell adherence and protection. Microbial biofilms produced by bacteria render most of currently available antibiotics ineffective by creating a “shield” through which antibiotics cannot penetrate through. To test a compound’s ability to penetrate bacterial biofilm, we provide the Minimum Biofilm Eradication Concentration (MBEC) assay as a service to our clients to test the test compound’s ability to penetrate bacterial biofilm.
The MBEC assay is used to determine the concentration of an antimicrobial agent that penetrates and eradicates bacterial biofilms. Emery Pharma offers the peg lid microplate-based MBEC assay, which has the advantage of high biofilm consistency and the ability to test a range of concentrations in one assay. In addition to the MBEC assay, Emery Pharma offers custom antibiofilm protocol development to find the solution that best fits the client’s needs.
Antibiotic Resistance Development Studies is used to characterize the ability of a microorganism to develop resistance to an antimicrobial agent, using both single and multi-step methods. Emery Pharma offers both single-step assays to determine the frequency of mutation against an antimicrobial agent, as well as multi-step serial passage of microbes in a serial passage model.
Cytotoxicity testing is used to determine the toxicity of a test compound on mammalian cells and are an important part in determining whether a compound may be a viable drug candidate. Emery Pharma offers both quantitative and qualitative assays, such as MTT and MEM elution assays, respectively.
Antimicrobial synergy testing by checkerboard assay is used to determine the effect on potency of a combination of antibiotics in comparison to their individual antimicrobial activities. Synergy studies can be further assessed by time-kill kinetic analysis to determine the rate of killing with the combinations.
U.S. Pharmacopeial Convention (USP) Methods:
At Emery Pharma, we are equipped to test products to ensure compliance with USP standards, in particular: Antimicrobial Effectiveness Testing (AET), USP <51>; Microbial Examination of Nonsterile Products: Microbial Enumeration Tests, USP <61>;Microbial Examination of Nonsterile Products: Tests for Specified Microorganisms, USP <62>.
Bacterial Isolation and Identification:
Emery Pharma offers microbial isolation service using selective media and culture conditions, and microbial identification through genetic identification (i.e. PCR and sequencing) and/or the Analytical Profile Index (API©) test system.
Post-Antibiotic Effect Assay:
Emery Pharma offers the post-antibiotic effect assay to determine the capacity of an antimicrobial drug to inhibit the growth of bacteria even after removal of the drug from the culture.