Microbiology and Cell Biology Laboratory Services

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“ Microbiology and cell biology laboratory expertise, from routine antimicrobial screening, to method development, to advanced Research & Development projects”

Emery Pharma offers a wide range of microbiology and cell biology services in our state-of-the-art laboratory with hundreds of bacterial strains including multidrug-resistant clinical isolates, ESKAPE pathogens, and multiple fungal strains available in our inventory for immediate testing. Our laboratory at Emery Pharma routinely performs microbiological testing and our Ph.D. scientists provide both recommendations for methods set up, such as suggesting microorganisms to test, and insightful and timely analysis of results.

Minimum Inhibitory (MIC) and Minimal Bactericidal (MBC) Concentration Testing

Minimum Inhibitory Concentration (MIC) assay is an assay used to test antimicrobial susceptibility and sensitivity. The MIC assay tests antimicrobial susceptibility and sensitivity by measuring inhibition of growth of the test microorganisms as a function of concentration of the test article. Our Microbiologists use disc diffusion, agar dilution, and broth microdilution Minimum Inhibitory Concentration (MIC) testing methods according to CLSI protocols to test a test article’s antimicrobial activity based on the concentration.

Minimum Bactericidal Concentration (MBC) and Minimal Fungicidal Concentration (MFC) are techniques used to determine the lowest concentration of an antimicrobial test article that results in a 99.9% reduction in the initial microbial density. A Minimum Bactericidal Concentration (MBC) test is normally performed after a Minimum Inhibitory Concentration (MIC) test to distinguish if the antimicrobial test compounds are bactericidal or bacteriostatic.

Time-Kill Kinetic Assay

Time-Kill Assays are used to assess a test object’s in vitro rate-of-kill in regards to a chosen set of test microorganisms.  Generally, a 3 Log10 reduction is considered the minimum level at which a product has significant killing activity against a particular test microorganism, while, as in the Minimal Bactericidal Concentration (MBC) test, bactericidal activity is defined as a 99.9% or greater killing efficacy at a specified time. Emery Pharma has successfully provided Time-Kill assays following the guidelines set by the CLSI.

Biofilm Testing

Biofilms are formed by microorganisms, including bacteria and fungi, for cell adherence and protection. Microbial biofilms produced by bacteria render most of currently available antibiotics ineffective by creating a “shield” through which antibiotics cannot penetrate through. To test a compound’s ability to penetrate bacterial biofilm, we provide the Minimum Biofilm Eradication Concentration (MBEC) assay as a service to our clients to test the test compound’s ability to penetrate bacterial biofilm.

Antibiotic Resistance Testing

As the number of cases of antibiotic resistance infection rises and new antimicrobial drugs in the pipeline decrease, profiling possible types of resistant strains that might develop or emerge in the clinic becomes an increasingly important tool for developing new antimicrobial compounds. At Emery Pharma, we perform this antibiotic resistance test using methods to determine the frequency of spontaneous antibiotic resistance profiling include serial passage techniques with step-wise increase in antibiotic concentration on solid or liquid media.

Cytotoxicity Testing

Cytotoxicity testing is used as an important cellular biology technique for preliminary screening of the toxicity of a test compound or for preforming quality control analysis. Emery Pharma offers the cytotoxicity analysis as a service using the following methods: Agarose Overlay, MEM Elution, Direct Contact Cytotoxicity, Indirect Contact Agar Diffusion Test, or MTT Assay.

Antimicrobial Synergy Study – Checkerboard Testing

Antimicrobial synergy testing by checkerboard assay determines the effect on potency of a combination of antibiotics in comparison to their individual antimicrobial activities, and is represented as the Fractional Inhibitory Concentration (FIC) index value. Emery Pharma is skilled at performing this microbiological service as well as providing full analysis of the results.

U.S. Pharmacopeial Convention (USP) Methods

At Emery Pharma, we are equipped to test products to ensure compliance with USP standards, in particular: Antimicrobial Effectiveness Testing (AET), USP <51>; Microbial Examination of Nonsterile Products: Microbial Enumeration Tests, USP <61>;Microbial Examination of Nonsterile Products: Tests for Specified Microorganisms, USP <62>.

Do you have a question and you need answers?  Call us at (888) 983-6379 (international clients: 1 (510) 899-8814) for a complementary initial consultation with one of our experienced Ph.D. scientists or to request a no-obligation quote.  We are experts in all facets of Cell and Microbiology, and Medicinal, Analytical and Bioanalytical chemistry testing, our experienced scientific staff can complete even your most complex testing project on-time and within budget.