LC-MS/MS Quantitation of Each Monoclonal Antibody from a Six-Component Cocktail Drug Product in Human Serum

Antibody cocktails complicate bioanalysis: each component must be measured individually, and ligand-binding assays often require high-quality anti-idiotypic reagents that are slow, expensive, or unavailable. In this poster, Emery Pharma scientists present a single LC-MRM-MS workflow, paired with trypsin digestion and a bottom-up peptide approach, that quantitates all six mAbs from a cocktail drug product in human serum simultaneously — with linearity of R² > 0.999 across 1–1500 µg/mL and LOQs as low as 0.1 µg/mL.

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What You Will Learn

The bottom-up peptide workflow used to capture all six mAbs in a single LC-MRM-MS run

Surrogate peptide selection criteria: BLAST uniqueness, cleavage site avoidance, and modification-prone residues

Specificity, matrix effect, linearity, and sensitivity results across all six antibodies

When LC-MRM-MS is the right alternative to ligand-binding assays for multi-API biotherapeutics

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