Fluorescence Detection Services
At Emery Pharma, we offer comprehensive fluorescence detection services to support your research, quality control, and regulatory requirements. With our experienced team and state-of-the-art technology, we provide precise, reliable data to ensure the success of your project.
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Case Studies
Case Study 1: Sensitive Detection of DNA Using Fluorescent Labels
Background: Detecting low concentrations of DNA in biological samples can be challenging due to background noise and limited sensitivity.
Application: In this example case study, a DNA fragment was labeled with SYBR Green, a fluorescent dye that specifically binds to double-stranded DNA. Using a fluorescence detector (excitation at 497 nm, emission at 520 nm), the DNA was analyzed in trace amounts within a complex biological matrix.
Results: The fluorescent label improved the limit of detection (LOD) to 10 pg/mL, providing over a 50-fold increase in sensitivity compared to UV absorbance.
Conclusion: The use of SYBR Green labeling and fluorescence detection significantly enhanced the sensitivity and specificity of DNA detection.
Case Study 2: Protein Detection Using FITC-Labeled BSA
Background: In protein analysis, detecting low-abundance proteins can be challenging with standard UV detection.
Application: In this example case study, Bovine Serum Albumin (BSA) was labeled with Fluorescein Isothiocyanate (FITC) and analyzed with a fluorescence detector (excitation at 488 nm, emission at 520 nm). The fluorescent labeling enhanced the visibility of BSA in plasma samples.
Results: The fluorescence detection increased sensitivity by 100-fold, improving the limit of detection (LOD) to 0.1 ng/mL compared to 10 ng/mL with UV detection.
Conclusion: Using FITC labeling with a fluorescence detector significantly improved BSA detection sensitivity.
Contact Us!
Ready to enhance your research with Emery Pharma's fluorescence detection services? Get in touch with our team today for a consultation or quote.
Key Services
Fluorescence Detection for Trace Compound Analysis
Method Development and Validation
This technique offers unparalleled sensitivity and selectivity, especially for compounds at low concentrations. Fluorescence detection is ideal for both naturally fluorescing compounds and those chemically modified to fluorescence.
Derivatization for Non-Fluorescent Compounds
Biomarker Analysis
For compounds that do not naturally fluoresce, we utilize advanced derivatization techniques, ensuring even trace amounts can be detected and quantified with precision.
Quantitative Bioanalysis
Quantitative Bioanalysis
Fluorescence detection is used in conjunction with our LC-MS and UPLC systems to provide quantitative analysis in various applications. Our expertise ensures accurate, high-sensitivity measurements in complex sample matrices.
Applications
Emery Pharma's fluorescence detection services are applicable in a wide range of sectors, including:
Pharmaceuticals
Critical in drug analysis, particularly in pharmacokinetics and bioanalysis. Our team uses derivatization techniques to ensure detection and quantification of even trace compounds.
Environmental Testing
Fluorescence detection allows for the identification of polyaromatic hydrocarbons (PAHs), key pollutants in environmental samples. Detecting these compounds at low levels helps ensure regulatory compliance.
Food & Water Safety
We utilize fluorescence to detect contaminants like pesticides and additives in food and water, as well as to verify the presence of important compounds like vitamins or lipids.
Biological Molecules
Used for the sensitive detection of DNA, RNA, and proteins, often with fluorescent labels for increased specificity
Metabolomics
In combination with our Orbitrap system, fluorescence detection helps identify and quantify fluorescent metabolites, providing comprehensive insights into metabolic pathways and interactions.
Why Choose Emery Pharma?
At Emery Pharma, our state-of-the-art fluorescence detection services provide highly sensitive and selective analysis for a wide range of applications. Utilizing cutting-edge instrumentation, we offer precise quantification of fluorescent compounds, ensuring accurate and reproducible results. Whether you need method development, validation, or custom assay design, our expert team is ready to support your analytical needs.
Contact us today to learn how our fluorescence detection capabilities can advance your research and product development.
Fast Turnaround Times
We know timely results are crucial, and we work efficiently to provide fast project completion without compromising on quality.
Industry Expertise
Our team has extensive experience in fluorescence-based techniques across various industries, ensuring reliable and accurate detection.
Tailored Solutions
Our fluorescence detection services are customizable to fit the unique requirements of your project, ensuring precise and relevant results
Comprehensive Support
From method development to final data reporting, we collaborate with you every step of the way to ensure your project's success.